| 货号 | 2352S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 使用方法 | WB(1:2000) IP (1:50) |
| 供应商 | CST |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from HeLa, NIH/3T3 and A431 cells, using MEK1 (61B2) Mouse mAb. | |
Western blot方法检测细胞提取物：HeLa, NIH/3T3和A431细胞系，使用的抗体是MEK1 (61B2) Mouse mAb鼠单抗。 |
