| 货号 | 6995S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Neurofibromatosis 2 (NF2) is an autosomal dominant inherited disorder that is characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors in the general population are caused by inactivation of the NF2 tumor suppressor gene. Merlin (moesin, ezrin, and radixin like protein), the NF2 gene product, has striking similarity to ezrin, radixin, and moesin (ERM). Regulation of merlin (aka schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from fetal rat brain, and both wild type and NF2(-/-) mouse embryonic fibroblasts (MEFs) using Merlin (D1D8) Rabbit mAb (upper) and Ezrin/Radixin/Moesin Antibody #3142 (lower). (MEF wt and MEF NF2(-/-) cells were kindly provided by Dr. Andrea McClatchey and Dr. Andrew Gladden, MGH Cancer Center and Harvard Medical School, Charlestown MA). Western blot分析胎大鼠，野生型和NF2(-/-)小鼠胚胎成纤维细胞（MEFs）提取物，使用的抗体是Merlin (D1D8) Rabbit mAb 兔单抗（上图）和Ezrin/Radixin/Moesin Antibody 兔多抗 #3142（下图）。（MEF野生型和MEF NF2(-/-)突变型由MGH Cancer Center and Harvard Medical School, Charlestown MA的Dr. Andrea McClatchey 和 Dr. Andrew Gladden提供。） |
