| 货号 | 12547S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | MetAP2 (D3I1H) Rabbit mAb recognizes endogenous levels of total MetAP2 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with MetAP1. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Eukaryotic initiation factor 2 (eIF2)-associated glycoprotein, p67/methionine aminopeptidase 2 (MetAP2) is one of the three known MetAPs responsible for the co-translational processing of the N-terminal initiator methionine from nascent proteins in cells. MetAP2 regulates the rates of global protein synthesis by controlling the levels of eIF2α phosphorylation (1). MetAP2 has also been shown to bind Erk1/2 to inhibit their activation and activity, thus connecting the protein synthesis machinery with the cell signaling pathway mediated by Erk1/2 MAP kinases (2-4). Although MetAP2 is characterized as having aminopeptidase activity that removes the N-terminal methionine from nascent peptides in vitro, mounting evidence suggests that MetAP2 has no methionine aminopeptidase activity. Rather, MetAP2 possesses auto-proteolytic activity that can be inhibited by several small molecule inhibitors including anti-angiogenic drugs, fumagillin and its derivatives (5). It has also been demonstrated that O-GlcNAcylation of MetAP2 plays a major role in its stability, eIF2α binding, and maintenance of eIF2α phosphorylation (6). MetAP2 knockout mice show embryonic lethality, suggesting its role in embryonic development and survival at the initiation of gastrulation (7). It is likely that lowering the levels of MetAP2 in mammalian cells causes cell growth inhibition and leads to apoptosis due to the high levels of eIF2α phosphorylation that inhibits global protein synthesis (8). During pathological or various stress conditions, MetAP2 dissociates from eIF2 subunits possibly due to its deglycosylation-induced autoproteolytic cleavage. As a result, eIF2α becomes hyperphosphorylated and global protein synthesis is inhibited. eIF2 complex-dissociated MetAP2 also displays a higher affinity toward Erk1/2, which results in the blockade of Erk1/2 activity. Thus, MetAP2 mediates cooperation between cell signaling and protein synthesis machinery to regulate cell growth and proliferation during physiological and pathological conditions (9). Research studies have shown higher expression of MetAP2 in human cancers, supporting the contention that MetAP2 plays a role in oncogenesis. For example, investigators have reported high MetAP2 expression in follicular lymphomas, large B-cell lymphomas, and Burkitts lymphomas (10). Elevated expression of MetAP2 has also been reported in human colorectal adenocarcinomas (11). |
| 存放说明 | -20C |
| 计算分子量 | 63 |
Western blot analysis of extracts from various cell lines using MetAP2 (D3I1H) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为MetAP2 (D3I1H) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human MetAP2 (hMetAP2-DDK; +), using MetAP2 (D3I1H) Rabbit mAb. Western blot方法检测293T细胞提取物，分别用空白对照（-）或构建的带有DDK标签的全长人MetAP2 (hMetAP2-DDK; +)转染细胞。使用的抗体为 MetAP2 (D3I1H) Rabbit mAb徒弟那爱看那个。 |
