| 货号 | 13557S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Mios (D12C6) Rabbit mAb recognizes endogenous levels of total Mios protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The mTORC1 kinase complex plays a critical role in cell growth regulation (1,2). mTORC1 activity is modulated by energy levels, growth factors, and amino acids (3,4). Four related GTPases (RagA, RagB, RagC, and RagD) interact with raptor in mTORC1, which is necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). The GAP activity towards Rags (GATOR) complex interacts with Rag GTPases and is made up of a pair of protein subcomplexes (5). The GATOR1 subcomplex includes the proteins DEPDC5, Nprl2 and Nprl3, and is a RagA and RagB GTPase-activating protein (GAP) that negatively regulates mTORC1 signaling. Conversely, the GATOR2 subcomplex (including Mios, WDR24, WDR59, Seh1L and Sec13 proteins) is a positive regulator of mTORC1 signaling (5). |
| 存放说明 | -20C |
| 计算分子量 | 98 |
Western blot analysis of extracts from various cell lines using Mios (D12C6) Rabbit mAb. 使用Mios (D12C6) Rabbit mAb兔单抗对多种细胞系提取物进行western blot分析。 | |
Immunoprecipitation of Mios from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP ® ; Isotype Control #3900 (lane 2) or Mios (D12C6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Mios (D12C6) Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 Mios (D12C6) Rabbit mAb (lane 3)对MCF7细胞中的Mios进行免疫沉淀实验。Lane1是10%上样量。使用Mios (D12C6) Rabbit mAb兔单抗进行western blot分析。 |
