| 货号 | 9482S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | Mitofusin-2 (D2D10) Rabbit mAb recognizes endogenous levels of total mitofusin-2 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Mitofusins are mitochondrial transmembrane GTPases that function to regulate mitochondrial fusion, a process that occurs in concert with mitochondrial division and is necessary for the maintenance of structural and genetic mitochondrial integrity (1,2). Two mitofusins have been described in mammals, mitofusin-1 and -2, which share 60% amino acid identity and appear to function coordinately to regulate mitochondrial fusion (3). Mitochondrial fusion is widely recognized as important for normal cell growth and development (4), and may have evolved as a mechanism to offset the deleterious effects of mtDNA mutations (3). Null mutations in either mitofusin are embryonic lethal in mice, whereas conditional knockout studies have shown that combined deletion of mitofusin-1 and mitofusin-2 in skeletal muscle results in severe mitochondrial dysfunction (3). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from various cell lines using Mitofusin-2 (D2D10) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为 Mitofusin-2 (D2D10) Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of HeLa (left) and NIH/3T3 (right) cells using Mitofusin-2 (D2D10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞（左）和NIH/3T3细胞（右），使用抗体为Mitofusin-2 (D2D10) Rabbit mAb兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白微丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Mitofusin-2 (D2D10) Rabbit mAb. 免疫组织化学分析石蜡包埋的人结肠癌切片，使用抗体为Mitofusin-2 (D2D10) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse heart using Mitofusin-2 (D2D10) Rabbit mAb. 免疫组织化学分析石蜡包埋的小鼠心脏，使用抗体为Mitofusin-2 (D2D10) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Mitofusin-2 (D2D10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组织化学分析石蜡包埋的人卵巢癌切片，对照肽（左）或抗原特异性肽（右），使用抗体为 Mitofusin-2 (D2D10) Rabbit mAb兔单抗。 |
