| 货号 | 12559S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | MRP2/ABCC2 (D9F9E) Rabbit mAb recognizes endogenous levels of total MRP2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family (1,2). The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs (2). MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate (3-5). MRP2 is predominately expressed on the apical membranes in the liver (6-9) and kidney proximal tubules (10). It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes (11-13). |
| 存放说明 | -20C |
| 计算分子量 | >200 |
Western blot analysis of extracts from Hep G2 and A-204 cells using MRP2/ABCC2 (D9F9E) Rabbit mAb. Western blot检测Hep G2和A-204细胞提取物，使用抗体为MRP2/ABCC2 (D9F9E) Rabbit mAb兔单抗。 | |
Immunoprecipitation of MRP2 from Hep G2 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or MRP2/ABCC2 (D9F9E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MRP2/ABCC2 (D9F9E) Rabbit mAb. 免疫沉淀分析Hep G2细胞提取物MRP2，使用 Normal Rabbit IgG #2729 (泳道 2) 或 MRP2/ABCC2 (D9F9E) Rabbit mAb 兔单抗(泳道3)。泳道1是10%对照。使用 MRP2/ABCC2 (D9F9E) Rabbit mAb兔单抗进行Western blot检测。 |
