| 货号 | 12705S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/IF-IC |
| 目标/特异性 | ABCC4 (D2Q2O) Rabbit mAb recognizes endogenous levels of total ABCC4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | ABCC4 is a member of the ATP-binding Cassette (ABC) transporter family. ABC proteins transport various molecules across cellular membranes by utilizing the energy generated from ATP hydrolysis. There are seven subfamilies of ABC proteins: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White (1). ABCC4 belongs to the MRP subfamily, which is involved in multi-drug resistance, hence it is also named MRP4. ABCC4 is widely expressed in tissues including prostate, kidney proximal tubules, astrocytes and capillary endothelial cells of the brain, platelets, and many cancer cell lines (2-4). ABCC4 mediates efflux transport of a wide variety of endogenous and xenobiotic organic anionic compounds (5). The diversity of substrates determines the biological functions of ABCC4. It regulates cAMP levels in human leukemia cells, thereby controlling the proliferation and differentiation of leukemia cells (6). ABCC4 also enables COX deficient pancreatic cancer cells to obtain exogenous prostaglandins (7). Research studies have shown that ABCC4 expression is elevated in drug resistant cancer cells, which makes it a potential target for cancer therapy (8,9). ABCC4 localizes to both plasma membrane and intracellular membranous structures (10). Investigators have also implicated ABCC4 in the pathogenesis of Kawasaki disease, a childhood genetic disorder characterized by vasculitis (11). |
| 存放说明 | -20C |
| 计算分子量 | 140-200 |
Confocal immunofluorescent analysis of HT-29 cells using ABCC4 (D2Q2O) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用ABCC4 (D2Q2O) Rabbit mAb (绿色)对HT-29细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Confocal immunofluorescent analysis of mouse kidney using ABCC4 (D2Q2O) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用ABCC4 (D2Q2O) Rabbit mAb (绿色)对小鼠肾脏组织进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin(红色)。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from SW620 and U-2 OS cells using ABCC4 (D2Q2O) XP®Rabbit mAb.使用ABCC4 (D2Q2O) XP® Rabbit mAb对SW620和U-2 OS细胞提取物进行western blot分析。 |
