| 货号 | 2850S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between non-identical DNA sequences and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH2 have been found in a large proportion of hereditary non-polyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations have also been associated with other sporadic tumors.DNA错配修复系统(MMR)能够修复复制后DNA，抑制非相同DNA序列间的重组，诱导伴随某些DNA损伤类型的检验点和凋亡反应(1)。MSH2 (MutS 同源蛋白2)和MSH6组成的hMutS-α二聚体是错配修复过程必不可少的部分。hMutS-α是BRCA1相关监视蛋白复合体(BRSC)的一部分，该复合体还包括BRCA1, MLH1, ATM, BLM, PMS蛋白和Rad50-Mre11-NBS1复合体(2)。在大部分遗传性非息肉性大肠癌（Lynch综合征）中已发现MSH2的突变，该疾病是西方国家最常见的遗传性结肠癌(3)。突变也与其它的散发性肿瘤相关。 |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Western blot analysis of extracts of HeLa and OVCAR3 cells using MSH2 (3A2) Mouse mAb.western blot方法检测 HeLa和OVCAR3细胞提取物，使用的抗体为 MSH2 (3A2) Mouse mAb。 | |
Confocal immunofluorescent analysis of HeLa cells using MSH2 (3A2) Mouse mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).激光共聚焦免疫荧光方法检测HeLa细胞，使用的抗体为MSH2 (3A2) Mouse mAb，呈绿色。肌动蛋白丝用DY554鬼笔环肽标记，呈红色。 |
