| 货号 | 12988S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IHC-P (1:500) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4). DNA错配修复系统（MMR）修复复制后DNA，抑制不同源DNA序列间的重组并导致某些DNA损伤后的检验点和凋亡反应（1）。MSH2 (MutS homologue 2) 会和MSH6形成hMutS-α二聚体，是错配修复过程的重要成员。hMutS-α是BRCA1相关监视复合物（BASC）的一部分，这个复合物还包括BRCA1, MLH1, ATM, BLM, PMS2蛋白以及Rad50-Mre11-NBS1复合物（2）。大部分的遗传性非息肉病性大肠癌(林奇综合征)病人，西方国家最常见的遗传性大肠癌，都发现有MSH6和其他MMR蛋白的突变（3）。MSH6突变也被发现在替莫唑胺疗法引发的恶性胶质瘤中，会促进替莫唑胺耐药性（4）。 |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Confocal immunofluorescent analysis of 293T (left) and HCT-15 (right) cells, using MSH6 (3E1) Mouse mAb (green) and β-Actin (D6A8) Rabbit mAb #8457 (red).使用MSH6 (3E1) Mouse mAb (绿色) 和β-Actin (D6A8) Rabbit mAb #8457 (红色)对293T（左）和HCT-15（右）进行激光共聚焦荧光分析。. | |
Immunohistochemical analysis of paraffin-embedded non-small cell lung carcinoma using MSH6 (3E1) Mouse mAb.使用MSH6 (3E1) Mouse mAb.对石蜡包埋的人非小细胞肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using MSH6 (3E1) Mouse mAb.使用 MSH6 (3E1) Mouse mAb对石蜡包埋的大肠癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, 293T (left) and HCT-15 (right) using MSH6 (3E1) Mouse mAb. 使用MSH6 (3E1) Mouse mAb对石蜡包埋的293T（左）和HCT-15（右）进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using MSH6 (3E1) Mouse mAb. 使用MSH6 (3E1) Mouse mAb对多种细胞提取物进行进行western blot分析。 | |
Western blot analysis of extracts from cell lines with varied expression of MSH6, using MSH6 (3E1) Mouse mAb (upper), MSH6 (D60G2) XP® Rabbit mAb #5424 (middle), or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).使用MSH6 (3E1) Mouse mAb.（上），MSH6 (D60G2) XP®Rabbit mAb #5424（中），或α-Actinin (D6F6) XP® Rabbit mAb #6487（下）对多种表达MSH6的细胞提取物进行western blot分析。 |
