MUC1 (VU4H5) Mouse mAb【科瑞奥博】

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MUC1 (VU4H5) Mouse mAb

货号： 4538S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	4538S
反应种属	Human
来源宿主	Mouse
应用	W/IHC-P/IF-IC/F
目标/特异性	MUC1 (VU4H5) Mouse mAb detects endogenous levels of total MUC1. This antibody does not cross-react with other mucin proteins.
使用方法	WB(1:1000) IHC-P (1:100) F (1:1600) IF-IC (1:100)

性能
供应商	CST
背景	Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrently overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a cytoplasmic tail of 72 residues (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with beta-catenin, which is regulated by GSK-3beta, PKCdelta and Src through phosphorylation at Ser44, Thr41 and Tyr46 of MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6), and attenuates stress induced apoptosis through the Akt or p53 pathways (7,8).
存放说明	-20C
计算分子量	230, 400

参考图片

Flow cytometric analysis of MCF-7 cells, using MUC1 (VU4H5) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).使用MUC1 (VU4H5)鼠单抗（蓝色）对MCF-7细胞进行流式分析，对照使用阴性对照抗体（红色）。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MUC1 (VU4H5) Mouse mAb.使用MUC1 (VU4H5)鼠单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MUC1 (VU4H5) Mouse mAb.使用MUC1 (VU4H5) 鼠单抗对石蜡包埋的人结肠癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using MUC1 (VU4H5) Mouse mAb.使用MUC1 (VU4H5) 鼠单抗对石蜡包埋人肺癌组织进行免疫组化分析。

Western blot analysis of extracts from PL45 and MCF-7 cells using MUC1 (VU4H5) Mouse mAb.使用 MUC1 (VU4H5) 鼠单抗uiPL45和MCF-7细胞提取物进行western blot分析。

Confocal immunofluorescent analysis of MCF7 and MDA-MB-231 cells using MUC1 (VU4H5) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用MUC1 (VU4H5) 兔单抗（绿色）对MCF7 和MDA-MB-231 细胞进行激光共聚焦荧光分析。肌动蛋白丝用鬼笔环肽DY-554标记。蓝色假色=DRAQ5® #4084 （DNA荧光染料）。