| 货号 | 4283S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | MyD88 (D80F5) Rabbit mAb detects endogenous levels of total MyD88 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm. |
| 存放说明 | -20C |
| 计算分子量 | 33 |
Western blot analysis of extracts from Raji, A549, and THP-1 cells using MyD88 (D80F5) Rabbit mAb.Western免疫印迹。用MyD88 (D80F5) Rabbit mAb研究 Raji, A549和THP-1细胞的细胞提取液。 | |
Western blot analysis of extracts from A549 cells, untransfected (—) or transfected with a MyD88 siRNA (+), using MyD88 (D80F5) Rabbit mAb.Western免疫印迹。用MyD88 (D80F5) Rabbit mAb研究未转染 (—) 和转染了MyD88 siRNA (+) A549细胞的细胞提取液。 |
