| 货号 | 8189S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
Confocal immunofluorescent analysis of COS-7 cells using Myosin IIc (D4A7) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Myosin IIc (D4A7) Rabbit mAb (绿色)和β-Actin (8H10D10) Mouse mAb #3700 (红色)标记，共聚焦免疫荧光分析COS-7细胞。蓝色= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from various cell lines using Myosin IIc (D4A7) Rabbit mAb.使用Myosin IIc (D4A7) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中Myosin IIa蛋白水平。 |
