| 货号 | 23565S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IHC-P (1:250) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9). |
| 计算分子量 | 100 |
Confocal immunofluorescent analysis of SK-MEL-28 (left), SNB19 (middle) or A-204 (right) cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from SK-MEL-28, SNB19 and A-204 cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Na,K-ATPase α1 (D4Y7E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human appendix using Na,K-ATPase α1 (D4Y7E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and A-204 (right) cell pellets using Na,K-ATPase α1 (D4Y7E) Rabbit mAb. |
