| 货号 | 4893S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Nanog (1E6C4) Mouse mAb detects endogenous levels of total nanog protein. |
| 使用方法 | WB(1:1000) IHC-P (1:12800) F (1:200) IF-IC (1:2000) |
| 供应商 | CST |
| 背景 | Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
Western blot analysis of extracts from NTERA and NCCIT cells using Nanog (1E6C4) Mouse mAb. | |
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (1E6C4) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).使用Nanog (1E6C4)鼠源单克隆抗体（绿色）对NTERA-2（左）和HeLa细胞（右）进行激光共聚焦荧光分析。用DY-554鬼笔环肽（红色）标记肌动蛋白丝。 | |
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb.使用Nanog（1E6C4）鼠源单克隆抗体对石蜡包埋的人精原细胞瘤进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells (red) and NTERA2 cells (blue) using Nanog (1E6C4) Mouse mAb.使用Nanog （1E6C4）鼠源单克隆抗体对HeLa细胞（红色）和NTERA2细胞（蓝色）进行流式细胞分析。 | |
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 (right).使用Nanog (1E6C4) 鼠源单克隆抗体对石蜡包埋的人精原细胞瘤进行免疫组化分析，生物素（左）或SignalStain® Boost 免疫组化检测试剂（HRP，小鼠）#8125（右）。 |
