货号 | 13473S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP |
使用方法 | WB(1:1000) IP (1:100) |
供应商 | CST |
背景 | The structural maintenance of chromosomes 2 (SMC2) and 4 (SMC4) proteins are condensin complex subunits that enable chromosome condensation and compaction during migration to opposite poles during anaphase (1,2). Condensin is a general regulator of chromosome architecture that may also regulate gene expression and DNA repair. Condensin complex subunits SMC2 and SMC4 form a functional ATPase essential for chromatin condensation, while three auxiliary subunits regulate ATPase activity. Both SMC2 and SMC4 are found within two distinct condensin complexes (condensin I and II) in higher eukaryotes. Condensin I contains auxiliary subunits NCAPD2, NCAPG, and NCAPH, while condensin II contains related auxiliary proteins NCAPD3, NCAPG2, and NCAPH2 (1,2). |
存放说明 | -20C |
计算分子量 | 170 |
Western blot analysis of extracts from HeLa and 293 cell lines using NCAPD3 (D3H6L) Rabbit mAb.使用NCAPD3 (D3H6L) Rabbit mAb对HeLa和293细胞提取物进行western blot分析。 | |
Immunoprecipitation of NCAPD3 from 293 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or NCAPD3 (D3H6L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NCAPD3 (D3H6L) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 NCAPD3 (D3H6L) Rabbit mAb (lane 3)对293细胞提取物中的NCAPD3进行免疫沉淀实验。Lane1是10%上样量。NCAPD3 (D3H6L) Rabbit mAb用作western blot分析。 |