| 货号 | 12943S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F |
| 目标/特异性 | NeuN (D3S3I) Rabbit mAb recognizes endogenous levels of total NeuN protein. A 30 kDa protein of unknown origin may be detected. |
| 使用方法 | WB(1:1000) IF-F (1:500) |
| 供应商 | CST |
| 背景 | Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3). |
| 存放说明 | -20C |
| 计算分子量 | 46-55 |
Confocal immunofluorescent analysis of normal mouse hippocampus (left), cortex (middle), and cerebellum (right) using NeuN (D3S3I) Rabbit mAb (green), and GFAP (GA5) Mouse mAb (Alexa Fluor®555 Conjugate) #3656 (red). Blue pseudocolor = DRAQ5® #4084 (Fluorescent DNA dye). 共聚焦免疫荧光分析正常小鼠海马(左)，皮质（中）和小脑（右），使用的抗体是NeuN (D3S3I) Rabbit mAb 兔单抗(绿色)， 和 GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (红色)。蓝色伪彩= DRAQ5® #4084 (荧光 DNA 染料)。 | |
