| 货号 | 4373S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | NeuroD (D35G2) Rabbit mAb detects endogenous levels of total NeuroD protein. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:50) |
| 供应商 | CST |
| 背景 | NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG (1,2). Neuronal activity results in CaMKII-mediated phosphorylation of NeuroD at Ser336, which is necessary for formation and growth of dendrites (3,4). NeuroD is also phosphorylated at Ser274 though the results are context dependent as phosphorylation by Erk stimulates NeuroD activity in pancreatic β-cells while phosphorylation by GSK-3β inhibits NeuroD in neurons (3). NeuroD is crucially important in both the pancreas and developing nervous system, and plays a large role in the development of the inner ear and mammalian retina (3). Mice lacking NeuroD become severely diabetic and die shortly after birth due to defects in β-cell differentiation (2,3,5,6). The lack of NeuroD in the brain results in severe defects in development (5). Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young (1,3). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
Western blot analysis of extracts from IMR-32 cells using NeuroD (D35G2) Rabbit mAb. Western blot分析IMR-32细胞提取物，使用的抗体是NeuroD (D35G2) Rabbit mAb 兔单抗。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 SH-SY5Y cells treated with Human Brain-Derived Neurotrophic Factor (BDNF) #3897 (50 ng/ml) for 30 minutes and either 10 μl of NeuroD (D35G2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human EGR1 Promoter Primers #5549, human EGR2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒，将Human Brain-Derived Neurotrophic Factor (BDNF) #3897 (50ng/ml)处理30分钟的4 x 10^6 SH-SY5Y细胞的染色质，与10 μl NeuroD (D35G2) Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用SimpleChIP® Human EGR1 Promoter Primers #5549、人EGR2启动子引物和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR定量富集的DNA。每个样本中免疫沉淀的DNA量用总投入量（等于1）的百分比表示。 |
