| 货号 | 2837S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-F |
| 目标/特异性 | Neurofilament-L (C28E10) Rabbit mAb detects endogenous levels of total Neurofilament-L protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-F (1:100) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinsons disease (in Lewy bodies along with α-synuclein), Alzheimers disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析正常大鼠小脑，使用Neurofilament-L (C28E10) Rabbit mAb 兔单抗（绿色）和GFAP (GA5) Mouse mAb 鼠单抗#3670（红色）进行标记。蓝色伪彩=DRAQ5® #4084 (荧光DNA染料)。 | |
Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb. Western blot分析小鼠大脑、HeLa细胞和大鼠大脑提取物，使用的抗体是Neurofilament-L (C28E10) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right). 免疫组化分析石蜡包埋的人大脑组织，使用的抗体是Neurofilament-L (C28E10) Rabbit mAb 兔单抗，其中左图存在对照肽，右图存在Neurofilament-L blocking peptide #1005。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb. 免疫组化分析石蜡包埋的小鼠大脑，使用的抗体是Neurofilament-L (C28E10) Rabbit mAb 兔单抗 。 |
