| 货号 | 2835S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-F |
| 目标/特异性 | Neurofilament-L (DA2) Mouse mAb detects endogenous levels of total Neurofilament-L protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-F (1:100) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinsons disease (in Lewy bodies along with α-synuclein), Alzheimers disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from rat brain, mouse brain, U87-MG cells, C6 cells and human cerebellum, using Neurofilament-L (DA2) Mouse mAb. Western blot分析大鼠大脑、小鼠大脑、U87-MG细胞、C6细胞和人小脑提取物，使用的抗体是Neurofilament-L (DA2)Mouse mAb 鼠单抗。 | |
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-L (DA2) Mouse mAb (green) and CREB (48H2) Rabbit mAb #9197 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 共聚焦免疫荧光分析小鼠小脑，使用Neurofilament-L (DA2) Mouse mAb 鼠单抗（绿）和 CREB (48H2) Rabbit mAb 兔单抗 #9197（红）进行标记。蓝色伪彩=DRAQ5™ (荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human brain, showing neuronal staining, using Neurofilament-L (DA2) Mouse mAb. 免疫组化分析石蜡包埋的人大脑，使用的抗体是Neurofilament-L (DA2) Mouse mAb 鼠单抗 ，显示神经元染色。 | |
Immunohistochemical analysis of paraffin-embedded human brain, showing neuronal staining, using Neurofilament-L (DA2) Mouse mAb. 免疫组化分析石蜡包埋的人脑，使用的抗体是Neurofilament-L (DA2) Mouse mAb 鼠单抗，显示神经元染色。 |
