| 货号 | 2838S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-F |
| 目标/特异性 | Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:25) IF-F (1:200) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinsons disease (in Lewy bodies along with α-synuclein), Alzheimers disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb. Western blot分析大鼠大脑（未处理，或λ磷酸酶处理）提取物，使用的抗体是Neurofilament-M (RMO 14.9) Mouse mAb 鼠单抗。 | |
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-M (RMO 14.9) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 共聚焦免疫荧光分析小鼠小脑，使用Neurofilament-M (RMO 14.9) Mouse mAb 鼠单抗（绿）和Calbindin Antibody 兔单抗#2136（红）进行标记。蓝色伪彩=DRAQ5 TM(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-M (RMO 14.9) Mouse mAb. 免疫组化分析石蜡包埋的人大脑，使用的抗体是Neurofilament-M (RMO 14.9) Mouse mAb 鼠单抗。 |
