| 货号 | 3017S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | NF-kappaB2 p100/p52 (18D10) Rabbit mAb detects endogenous levels of both p100, the precursor, and p52 protein, the active form of NF-kappaB2. The antibody does not cross-react with other family members. |
| 使用方法 | WB(1:1000) IHC-P (1:300) F (1:50) |
| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 52 active form. 120 precursor. |
Flow cytometric analysis of HeLa cells, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific) (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究 HeLa 细胞。所用抗体为NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific) (蓝色)与非特异性的阴性抗体对比(红色)。 | |
Western blot analysis of extracts from HeLa, and COS cells, using NF-kB2 p100/p52 (18D10) Rabbit mAb (Human Specific).Western免疫印迹。用NF-kB2 p100/p52 (18D10) Rabbit mAb (Human Specific)研究HeLa和COS 细胞的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded human osteosarcoma, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific).免疫组织化学染色分析石蜡包埋人骨肉瘤组织。所用抗体为NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific).免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为NF-kappaB2 p100/p52 (18D10) Rabbit mAb (Human Specific)。 |
