| 货号 | 3447S |
| 反应种属 | Human/Mouse/Rat/Bovine |
| 来源宿主 | Rat IgG2b |
| 应用 | W/IP |
| 目标/特异性 | Notch1 (5B5) Rat mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) protein and the transmembrane/intracellular region NTM (~120 kDa). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 120, 300 |
Western blot analysis of extracts from A2780 and A204 cells using Notch1 (5B5) Rat mAb. The transmembrane/intracellular region (NTM) is indicated. 使用Notch1（5B5）兔单抗对A2780和A204细胞提取物进行western blot分析。跨膜/细胞内区域如图所示。 |
