| 货号 | 2420S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Notch2 (8A1) Rabbit mAb detects endogenous levels of total Notch2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 230, 300 |
Western blot analysis of extracts from SNB19 and 293 cells, untreated or stably expressing human Notch2, using Notch2 (8A1) Rabbit mAb. The full-length (FL) and the extracellular domain (EC) of Notch2 are indicated. 使用Notch2 (8A1) 兔单抗对未处理或稳定表达人源Notch2蛋白的SNB19和293细胞提取物，进行western blot分析。Notch2蛋白全长（FL）和细胞外组分（EC）如图所示。 | |
Immunoprecipitation of endogenous Notch2 from 8MGBA cell lysates using Notch2 (8A1) Rabbit mAb. Lane 1 contains lysate input and lane 2 was immunoprecipitated with Notch2 (8A1) Rabbit mAb. Western blot detection was performed using the same antibody. 使用Notch2（8A1）兔单抗对8MGBA细胞提取物内源性Notch2进行免疫共沉淀实验。第一道加入裂解物，第二道为经过Notch2 (8A1) 兔单抗免疫共沉淀后样品。 |
