| 货号 | 2423S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | Notch4 (L5C5) Mouse mAb detects levels of the transfected intracellular region of human Notch4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 80 cleaved. 230 full-length. |
Western blot analysis of the recombinant human ankyrin repeat domains of Notch1, 2, 3 and 4 (HANK1-4, lanes 1-4, respectively), using 5 ng of protein per lane and Notch4 (L5C5) Mouse mAb. 使用Notch4（L5C5）Mouse mAb小鼠单抗对重组人Notch1，2,3 和4的ankyrin重复结构域（HANK1-4，1-4泳道）进行western blot分析，每个泳道加入5ng蛋白。 | |
Western blot analysis of total cell lysate of COS cells either untransfected or transiently transfected with a construct expressing the intracellular domain of human Notch4 (ICN4), using Notch4 (L5C5) Mouse mAb. 对未转染或瞬转了人Notch4（ICN4）细胞内区域表达质粒的COS细胞，使用Notch4（L5C5）小鼠单抗进行对获取的全细胞提取物进行western blot分析。 |
