| 货号 | 5276S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Notch3 (D11B8) Rabbit mAb detects endogenous levels of total Notch3 protein. The antibody recognizes both full-length (FL) Notch3 at 270 kDa and a truncated protein (NTM) containing a short extracellular region, the transmembrane domain and the intracellular region at 90 kDa. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 90, 270 |
Western blot analysis of extracts from various cell lines using Notch3 (D11B8) Rabbit mAb. 使用Notch3（D11B8）Rabbit mAb兔单抗对多种细胞提取物进行Western blot分析。 |
