| 货号 | 3187S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | NQO1 (A180) Mouse mAb detects endogenous levels of total NQO1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turns leads to myeloid hyperplasia of bone marrow (2). |
| 存放说明 | -20C |
| 计算分子量 | 29 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using NQO1 (A180) Mouse mAb. 免疫组织化学检测石蜡包埋的人结肠癌组织，所用抗体为NQO1 (A180) Mouse mAb鼠单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NQO1 (A180) Mouse mAb. 免疫组织化学检测石蜡包埋的人肺癌组织，所用抗体为NQO1 (A180) Mouse mAb鼠单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using NQO1 (A180) Mouse mAb. 免疫组织化学检测石蜡包埋的人乳腺癌组织，所用抗体为NQO1 (A180) Mouse mAb鼠单抗。 | |
Western blot analysis of extracts from several cell lines using NQO1 (A180) Mouse mAb. Wetern blot检测多种细胞系提取物，所用抗体为NQO1 (A180) Mouse mAb鼠单抗。 | |
Confocal immunofluorescent analysis of HeLa (left) and NIH/3T3 cells (right) labeled with NQO1 (A180) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测HeLa (左） 和 NIH/3T3 细胞 (右)，所用抗体为NQO1 (A180) Mouse mAb鼠单抗（绿），微丝用Alexa Fluor＠ 555 phalloidin (红)着色，蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084)。 |
