| 货号 | 14766S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1, Noxa) is a small protein that plays a key role in mediating apoptotic signaling. Noxa is a pro-apoptotic Bcl-2 family protein that contains a single Bcl-2 homology (BH3) domain (1). Members of the “BH3-only” family (e.g. Noxa, Bad, Bim, Puma, Bid, Bik, and Hrk) are highly regulated proteins that induce apoptosis through BH3-dependent interaction with anti-apoptotic Bcl-2 family proteins (2). Noxa localizes to mitochondria and binds the anti-apoptotic proteins Mcl-1 and A1/Bfl-1, but does not bind to Bcl-2 or Bcl-xL (3). The Noxa protein competes with Mcl-1 for binding to mitochondrial Bak protein. Noxa was originally identified as a phorbol ester inducible protein that is highly expressed in adult T-cell leukemia cell lines (4). Several different stimuli, including DNA damage, hypoxia, interferon, viral infection, and double-stranded RNA, induce Noxa expression in cells. Higher levels of Noxa protein are typically found hematopoietic cells (3,5,6). |
| 存放说明 | -20C |
| 计算分子量 | 10 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Noxa protein (hNoxa; +), using Noxa (D8L7U) Rabbit mAb. | |
Western blot analysis of extracts from serum starved Jurkat cells, untreated (-) or treated with TPA #4174 (10 ng/ml, 3 hr; +), using Noxa (D8L7U) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from KARPAS-299, RL-7, and L-540 cells lines using Noxa (D8L7U) Rabbit mAb. Cell Line Source: Dr. Abraham Karpas at the University of Cambridge. | |
Immunoprecipitation of Noxa protein from KARPAS-299 cells using Rabbit (DA1E) mAb XP® Isotype Control #3900 (lane 2) or Noxa (D8L7U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Noxa (D8L7U) Rabbit mAb. Cell Line Source: Dr. Abraham Karpas at the University of Cambridge. |
