| 货号 | 13160S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | NT5E/CD73 (D7F9A) Rabbit mAb recognizes endogenous levels of total NT5E/CD73 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 背景 | Ecto-5-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Flow cytometric analysis of BALB/c splenocytes using a CD3 antibody and Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left) or NT5E/CD73 (D7F9A) Rabbit mAb (right). Anti-rabbit IgG (H+L), F(ab)2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. | |
流式细胞分析BALB/c脾细胞，使用的抗体是CD3 antibody 兔多抗和Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (左)或 NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗 (右)。Anti-rabbit IgG (H+L), F(ab)2Fragment (PE Conjugate) #8885用作二抗。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with cobalt chloride (CoCl2, 0.1 mM, 16 hr; +), using NT5E/CD73 (D7F9A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
