| 货号 | 3053S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC |
| 目标/特异性 | NTF2 (5A3) Mouse mAb detects endogenous levels of total NTF2 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The small GTPase Ran resides on both the cytosolic and nucleosolic sides of the nuclear pore complex (NPC) and regulates the import and export of various proteins to and from the nucleus. Like other small GTPases, Ran exists in either a GTP-bound or GDP-bound state. RanGTP that resides in the nucleus and promotes nuclear export, while cytosolic RanGDP promotes import. The gradient of RanGTP across the nuclear membrane allows for appropriate movement of cargo proteins across the NPC as well as maintenance of the mitotic spindle (1-3). Nuclear transport factor 2 (NTF2) regulates the subcellular distribution and function of Ran (4-5). The NTF2 homodimer facilitates the diffusion of RanGDP through NPCs via transient interactions with phenylalanine-glycine (FG) repeat domains on NPC proteins. NTF2 stabilizes the GDP-bound form of Ran until it is induced to dissociate by a nuclear factor in an ATP-dependent manor, thus allowing the guanine nucleotide exchange factor (GEF) RCC1 to mediate exchange of GDP for GTP on Ran (6-7). |
| 存放说明 | -20C |
| 计算分子量 | 14 |
Western blot analysis of extracts from various cell types using NTF2 (5A3) Mouse mAb. 使用NTF2 (5A3) Mouse mAb，免疫印迹（Western Blot）分析不同细胞中NTF2 (5A3)蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using NTF2 (5A3) Mouse mAb (green). Actin filaments have been labeled with DY554 phalloidin (red). 使用NTF2 (5A3) Mouse mAb(绿色)，共聚焦免疫荧光分析HeLa细胞。DY554 phalloidin标记微丝蛋白(红色)。 |
