| 货号 | 3625S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F |
| 目标/特异性 | NUT (C52B1) Rabbit mAb detects endogenous levels of total NUT protein. The antibody also detects endogenous levels of the BRD4-NUT fusion protein found in NUT midline carcinoma (NMC). |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:45) IF-F (1:1600) |
| 供应商 | CST |
| 背景 | Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the NUT gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
Confocal immunofluorescent analysis of rat testes using NUT (C52B1) Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 在NUT midline carcinoma (NMC)中使用NUT (C52B1) Rabbit mAb (绿色)和Pan-Keratin (C11) Mouse mAb #4545 (红色)，共聚焦免疫荧光分析大鼠睾丸组织。蓝色= DRAQ5® #4084 (DNA荧光染料)。 | |
Immunohistochemical analysis of paraffin-embedded human midline carcinoma using NUT (C52B1) Rabbit mAb. 使用NUT (C52B1) Rabbit mAb，免疫组化分析人源中线癌石蜡切片。 | |
Immunohistochemical analsysis of paraffin-embedded human midline carcinoma using NUT (C52B1) Rabbit mAb. 使用NUT (C52B1) Rabbit mAb，免疫组化分析人源中线癌石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded normal human testes using NUT (C52B1) Rabbit mAb. 使用NUT (C52B1) Rabbit mAb，免疫组化分析人源睾丸组织石蜡切片。 | |
Western blot analysis of extracts from rat and human testis using NUT (C52B1) Rabbit mAb. 使用NUT (C52B1) Rabbit mAb，免疫印迹(Western blot)分析大鼠和人源睾丸组织中NUT (C52B1)的蛋白水平。 | |
Western blot analysis of extracts from rat testis and NUT midline carcinoma (NMC) cells using NUT (C52B1) Rabbit mAb. 使用NUT (C52B1) Rabbit mAb，免疫印迹(Western blot)分析大鼠睾丸和NUT中线癌(NMC)细胞中NUT (C52B1)蛋白水平。 |
