| 货号 | 8157S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | Oct-1 (D7B6) Rabbit mAb recognizes endogenous levels of total Oct-1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Westerrn blot analysis of extracts from various cell lines using Oct-1 (D7B6) Rabbit mAb. 使用Oct-1 (D7B6) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 minutes and either 10 μl of Oct-1 (D7B6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
