| 货号 | 67589S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape and have been shown to dramatically impact mitochondrial metabolism, apoptosis, and autophagy (1). These processes are largely controlled by mitochondrial dynamin-related GTPases, including mitofusin-1, mitofusin-2, OPA1, and DRP1. DRP1 regulates mitochondrial fission, while the mitofusins and OPA1 control fusion at the outer and inner mitochondrial membrane, respectively. |
| 存放说明 | -20C |
| 计算分子量 | 80-100 |
Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® OPA1 siRNA II (right), using OPA1 (D7C1A) Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® OPA1 siRNA I (+), or SignalSilence® OPA1 siRNA II using OPA1 (D7C1A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The OPA1 (D7C1A) Rabbit mAb confirms silencing of OPA1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. | |
Immunoprecipitation of OPA1 from MCF7 cell extracts. Lane 1 represents 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is OPA1 (D7C1A) Rabbit mAb. Western blot was performed using OPA1 (D7C1A) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG. | |
Western blot analysis of extracts from MCF7 or HeLa cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP; +) using OPA1 (D7C1A) Rabbit mAb. |
