| 货号 | 4736S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rat IgG2a |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).起始识别复合物(ORC)是一种高度保守的异源六聚化蛋白复合体，它与DNA复制起始位点处或其附近的DNA相关。ORC的六个亚基是DNA复制起始必不可少的(1-3)，而且ORC可能参与应激响应过程中的基因表达(4)。ORC结合DNA后允许其它蛋白如cdc6和MCMs的有序结合，从而形成前复制复合体(Pre-RCs)。Pre-RCs形成于细胞周期的末期和G1早期之间并伴随DNA合成的发生而被失活，允许了DNA复制和细胞分化的协同调节(5)。ORC单个或多个亚基的修饰可能是其S期失活的主要原因，而细胞分化阶段ORC亚基的染色质结合行为仍处于研究之中(6-7)。 |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from HeLa and HUVEC cells, using ORC2 (3G6) Rat mAb.Western blot 方法检测 HeLa 和 HUVEC细胞提取物，使用的抗体为ORC2 (3G6) Rat mAb。 | |
Immunoprecipitation of ORC2 from Jurkat cell lysates, using ORC2 (3G6) Rat mAb followed by western blot using the same antibody.从Jurkat细胞裂解物中免疫沉淀ORC2蛋白，使用的抗体为ORC2 (3G6) Rat mAb，随后用同一抗体进行western blot检测 。 | |
Confocal immunofluorescent analysis of HeLa cells using ORC2 (3G6) Rat mAb (blue) and COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测HeLa细胞，使用的抗体为ORC2 (3G6) Rat mAb (蓝色) 和 COX IV (3E11) Rabbit mAb #4850 (绿色). 肌动蛋白纤维使用DY-554鬼笔环肽标记，呈红色。 |
