| 货号 | 12497S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | OS-9 (D8P4G) Rabbit mAb recognizes endogenous levels of total OS-9 protein. This antibody reacts with OS-9 isoform 1 (OS-9-1) and OS-9 isoform 2 (OS-9-2). |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Amplified in Osteosarcoma 9 (OS-9) was identified as a gene that is frequently amplified in human sarcomas (1). OS-9 is an N-glycosylated ER resident protein that harbors a mannose-6-phosphate receptor homology (MRH) domain and plays a critical role in mammalian ER quality control, in part, by functioning as a lectin. There are two major isoforms of OS-9 (OS-9-1, OS-9-2) that are generated through alternative splicing of mRNA (2). The expression of both OS-9 isoforms is enhanced upon activation of the IRE1/XBP1 pathway in cells exposed to acute ER stress. It is postulated that OS-9 serves as a retention factor for glycosylated and nonglycosylated, misfolded proteins, preventing their progression through the secretory pathway (3,4). Furthermore, OS-9 promotes the removal of misfolded proteins from the ER lumen and their subsequent proteasome-dependent degradation via its association with the Hrd1-SEL1L ubiquitin ligase complex (5). |
| 存放说明 | -20C |
| 计算分子量 | 83,97 |
Western blot analysis of extracts from various cell lines using OS-9 (D8P4G) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为OS-9 (D8P4G) Rabbit mAb兔单抗。 | |
Immunoprecipitation of OS-9 from SJSA-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or OS-9 (D8P4G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed with OS-9 (D8P4G) Rabbit mAb. 免疫沉淀法检测SJSA-1细胞提取物中的OS-9，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（泳道2）或OS-9 (D8P4G) Rabbit mAb (泳道3)。泳道1为10% input对照。 Western blot 检测中使用的是OS-9 (D8P4G) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or thapsigargin-treated (2 nM, 16 hr; +), using OS-9 (D8P4G) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Western blot方法检测HeLa细胞提取物，未经处理（-）或thapsigargin处理 (2 nM, 16小时; +)，使用的抗体为 OS-9 (D8P4G) Rabbit mAb兔单抗 (上图) 或 GAPDH (D16H11) XP® Rabbit mAb兔单抗 #5174 (下图)。 |
