OTUB1 (D8F7) Rabbit mAb
货号：
3783S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 3783S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | OTUB1 (D8F7) Rabbit mAb recognizes endogenous levels of total OTUB1 protein. This antibody does not cross-react with OTUB2 and based upon sequence alignment, is not predicted to cross-react with OTUB1, isoform 2 (ARF-1). |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM (1,2). The OTU subfamily comprises a group of approximately 100 putative cysteine proteases that are homologous to the ovarian tumor gene product of Drosophila (3). OTUB1 and OTUB2 (OTU domain-containing Ubal-binding proteins) display no significant similarity to any known DUB, but are close homologs and possess an OTU domain that contains conserved cysteine, histidine, and aspartate residues that define the putative catalytic triad of cysteine proteases. Furthermore, sequence analysis of OTUB1 and OTUB2 reveals the presence of putative Ub-interaction motifs (UIMs) and Ub-associated domains (UBAs), which are characteristic of proteins that regulate protein ubiquitination. OTUB1 and OTUB2 also possess a putative nuclear localization signal (NLS) and a consensus LxxLL motif, which mediates the interaction between transcriptional co-activators and nuclear hormone receptors (4). |
| 存放说明 | -20C |
| 计算分子量 | 31 |
参考图片Western blot analysis of extracts from various cell lines using OTUB1 (D8F7) Rabbit mAb. Western blot检测多种细胞提取物。 |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with Myc/DDK-tagged cDNA expression constructs encoding full-length human OTUB1, isoform1 (hOTUB1-Myc/DDK, +) and full-length human OTUB2 (hOTUB2-Myc/DDK, +), using OTUB1 (D8F7) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (lower). Western blot检测mock transfected (-) 或转染表达Myc/DDK-tagged cDNA 编码人全长OTUB1 isoform1 (hOTUB1-Myc/DDK, +) 和人全长OTUB2 (hOTUB2-Myc/DDK, +)的293T细胞。采用OTUB1 (D8F7) Rabbit mAb (upper) 和DYKDDDDK Tag Antibody(lower)与Sigmas Anti-FLAG® M2 Antibody) #2368结合相同的表位。 |
