| 货号 | 13846S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | p130 Cas (Crk-associated substrate) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) (1) and the FAK-related kinase PYK2 (2), as well as the tyrosine phosphatases PTP-1B (3) and PTP-PEST (4). The carboxy-terminal Src binding domain (SBD) contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr668 and/or Tyr670) that can promote interaction with the SH2 domain of SFKs (5). The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr165, 249, and 410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains including adaptors, C-Crk, Nck, and inositol 5-phosphatase 2 (SHIP2) (6). The tyrosine phosphorylation of p130 Cas has been implicated as a key signaling step in integrin control of normal cellular behaviors including motility, proliferation, and survival. Aberrant Cas tyrosine phosphorylation may contribute to cell transformation by certain oncoproteins (5). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Western blot analysis of extracts from various cell lines using p130 Cas (E1L9H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of p130 Cas from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p130 Cas (E1L9H) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using p130 Cas (E1L9H) Rabbit mAb. | |
Confocal immunofluorescent analysis of SNB19 cells either untreated (left) or treated with TPA #9905 (100nM, 1hr; right) using p130 Cas (E1L9H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of HT-1080 cells (green) and Jurkat cells (blue) using p130 Cas (E1L9H) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p130 Cas (E1L9H) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using p130 Cas (E1L9H) Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right). |
