| 货号 | 2947S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).肿瘤抑制蛋白p21 Waf1/Cip1能够充当细胞周期进程抑制剂。其功能与周期蛋白和周期蛋白依赖性激酶形成的异源三聚体存在化学计量关系。通过与CDK2复合体联合，它可以抑制激酶的活性和阻断G1/S进程(1)。然而，p21也可以增强CDK4或者CDEK6与周期蛋白-D形成的复合体的组装和活性(2)。p21的羧基末端区域能够充分结合并抑制PCNA，DNA聚合酶的一个亚基，而且可以协调细胞周期进程中DNA的复制(3)。基于紫外线损伤或者是在细胞周期中cdc2/cyclin B或CDK2/cyclin A活跃阶段，p53被磷酸化且通过p53-响应元件上调p21的表达(4)。泛素化和蛋白酶体降解能够下调p21的蛋白水平(5)。 |
| 存放说明 | -20C |
| 计算分子量 | 21 |
Western blot analysis of extracts from various cell types using p21 Waf1/Cip1 (12D1) Rabbit mAb.Western blot方法检测不同细胞提取物，使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb。 | |
Immunoprecipitation of p21 from human umbillical vein endothelial cells (HUVECs) using p21 Waf1/Cip1 (12D1) Rabbit mAb. Western blot detection was performed using the same antibody.从HUVEC细胞免疫沉淀p21蛋白，使用p21 Waf1/Cip1 (12D1) Rabbit mAb抗体。Western blot检测使用同一抗体。 | |
Confocal immunofluorescent analysis of MCF-7 cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (red) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测MCF-7细胞，使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb，呈红色，和 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706，呈绿色。蓝色伪彩为DNA荧光染料，产品信息为DRAQ5® #4084 。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using p21 Waf1/Cip1 (12D1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学方法检测人类乳腺癌组织切片，使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb，左图为对照多肽共孵育，右图为抗原特异性多肽共孵育。 | |
Flow cytometric analysis of HeLa cells (red) and MCF-7 cells (blue), using p21 Waf1/Cip1 (12D1) Rabbit mAb.流式细胞术检测Hela细胞（红色），和MCF-7细胞（蓝色），使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left) or SignalSilence® p21 Waf1/Cip1 siRNA II #6558 (right), using p21 Waf1/Cip1 (12D1) Rabbit mAb.免疫组织化学检测石蜡包埋Hela细胞，分别转染SignalSilence® Control siRNA (Unconjugated) #6568 (左图) 和 SignalSilence® p21 Waf1/Cip1 siRNA II #6558 (右图), 使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® p21 Waf1/Cip1 siRNA II (+), using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 and α-Tubulin (11H10) Rabbit mAb #2125. The p21 Waf1/Cip1 (12D1) Rabbit mAb confirms silencing of p21 Waf1/Cip1 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p21 Waf1/Cip1 siRNA.Western blot方法分析Hela细胞提取物。分别用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或 SignalSilence® p21 Waf1/Cip1 siRNA II (+)转染细胞。使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb #2947和α-Tubulin (11H10) Rabbit mAb #2125。和对照抗体p42 monoclonal antibody相比，p21 Waf1/Cip1 monoclonal antibody的检测结果证实p21的表达被p21 Waf1/Cip1 siRNA特异性沉默。 |
