| 货号 | 12164S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | p190-A RhoGAP (D8Q6C) Rabbit mAb recognizes endogenous levels of total p190-A RhoGAP protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Rho family GTPases are key regulators of diverse processes such as cytoskeletal organization, cell growth and differentiation, transcriptional regulation, and cell adhesion/motility. The activities of these proteins are controlled primarily through guanine nucleotide exchange factors (GEFs) that facilitate the exchange of GDP for GTP, promoting the active (GTP-bound) state, and GTPase activating proteins (GAPs) that promote GTP hydrolysis and the inactive (GDP-bound) state (1,2). The p190 RhoGAP proteins are widely expressed Rho family GAPs. p190-A has been characterized as a tumor suppressor, and research studies have shown that loss or rearrangement of the chromosomal region containing the gene for p190-A is linked to tumor development (3,4). p190-A binds the mitogen-inducible transcription factor TFII-I, sequestering it in the cytoplasm and inhibiting its activity. Phosphorylation of p190-A at Tyr308 reduces its affinity for TFII-I, relieving the inhibition (5). p190-A can also inhibit growth factor-induced gliomas in mice (6) and affect cleavage furrow formation and cytokinesis in cultured cells (7). Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB (8). Cells deficient in p190-B display defective adipogenesis (9). There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain (9-11). Levels of tyrosine phosphorylation are enhanced by Src overexpression (10,11). IGF-I treatment downregulates Rho through phosphorylation and activation of p190-B RhoGAP, thereby enhancing IGF signaling implicated in adipogenesis (9). |
| 存放说明 | -20C |
| 计算分子量 | 190 |
Immunoprecipitation of p190-A RhoGAP from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p190-A RhoGAP (D8Q6C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using p190-A RhoGAP (D8Q6C) Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或p190-A RhoGAP (D8Q6C) 兔mAb (lane 3)对Jurkat细胞提取物中的p190-A RhoGAP进行免疫沉淀实验。Lane1是10%上样量。使用p190-A RhoGAP (D8Q6C) Rabbit mAb进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using p190-A RhoGAP (D8Q6C) Rabbit mAb.使用p190-A RhoGAP (D8Q6C) Rabbit mAb对多种细胞提取物进行western blot分析。 |
