| 货号 | 4696S |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/Zebrafish/Bovine/Pig |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:2000) IHC-P (1:500) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 42, 44 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic and nuclear localization, using p44/42 MAP Kinase (L34F12) Mouse mAb. | |
免疫组织化学方法检测石蜡包埋的人结肠癌组织，图中显示了细胞质和细胞核的位置。使用的抗体为p44/42 MAP Kinase (L34F12) Mouse mAb鼠单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p44/42 MAP Kinase (L34F12) Mouse mAb in the presence of control peptide (left) or p44/42 MAP Kinase Blocking Peptide (#4696 Specific) #1245 (right). | |
免疫组织化学方法检测石蜡包埋的人乳腺癌组织。使用的抗体为p44/42 MAP Kinase (L34F12) Mouse mAb鼠单抗，左侧在抗体中加入了对照组肽段，右侧在抗体中加入了p44/42 MAP Kinase Blocking Peptide (#4696 Specific) #1245。 | |
Western blot analysis of extracts from NIH/3T3, PC12 and COS cells, using p44/42 MAP Kinase (L34F12) Mouse mAb. |
