| 货号 | 4726S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rat IgG2a |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:250) |
| 供应商 | CST |
| 背景 | Initiation of eukaryotic DNA replication is a stringently regulated process that requires the cooperation of many proteins and protein complexes to occur efficiently, at the origins of replication, and once per cell cycle. The initiation of DNA replication requires a protein complex composed of two DNA polymerase α subunits and a pair of primase subunits. Primase activity catalyzes de novo synthesis of an RNA/DNA primer (initiator DNA) on the leading and lagging strands, while polymerase activity extends the initiator DNA (1). The 48 and 58 kDa primase subunits cooperate in the synthesis of small RNA primers. p48 is the catalytically active subunit (2), while p58 couples p48 to the polymerase to allow the transfer of primers to the active site. The p58 subunit may also play a role in regulation of primer length (3,4).真核生物DNA复制起始是一个严格控制的过程，需要在复制起始的时候多种蛋白质和蛋白复合体的协同作用高效率的发生，而且每个周期仅有一次。DNA复制的起始需要由两个DNA聚合酶α亚基组成的蛋白复合体和一对引物酶亚基。引物酶的活性催化前导链和后随链上的RNA/DNA引物(起始DNA)的从头合成，聚合酶的活性是延伸起始DNA(1)。在小的RNA引物的合成过程中48和58 kDa的引物酶亚基协同作用。p48是活化的催化亚基(2)，而p58藕连p48至聚合酶促进了引物向活化位点转移。P58亚基也在引物长度调节的过程中起作用(3,4)。 |
| 存放说明 | -20C |
| 计算分子量 | 58 |
Western blot analysis of extracts from various cell types using p58 Primase (8D3) Rat mAb.Western blot 方法检测不同细胞提取物，使用的抗体为p58 Primase (8D3) Rat mAb。 | |
Immunoprecipitation of p58 primase from HeLa cell lysates using p58 Primase (8D3) Rat mAb. Western blot analysis was performed using the same antibody.使用p58 Primase (8D3) Rat mAb从Hela细胞裂解液中免疫沉淀p58 Primase 。Western blot使用同一抗体检测。 |
