| 货号 | 48818S |
| 反应种属 | Human |
| 来源宿主 | Mouse IgG2b |
| 应用 | W/IHC-P/IF-IC/F/ChIP |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:50) IF-IC (1:800) ChIP (1:100) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 53 |
Confocal immunofluorescent analysis of untreated HT-29 cells (top left), untreated Saos-2 cells (top right), untreated MCF7 cells (bottom left), and MCF7 cells treated with Doxorubicin #5927 (0.5 μM, 24 hr; bottom right), using p53 (DO-7) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Flow cytometric analysis of H1299 cells (blue) and HT-29 cells (green) using p53 (DO-7) Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded HT-29 (left) and Saos-2 (right) cells using p53 (DO-7) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using p53 (DO-7) Mouse mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells treated with UV (100 J/m2 followed by a 3 hr recovery) and either 5 μl of p53 (DO-7) Mouse mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using p53 (DO-7) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Doxorubicin #5927 (0.5 μM, 24 hr; +), using p53 (DO-7) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using p53 (DO-7) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p53 (DO-7) Mouse mAb. |
