| 货号 | 9643S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PA28α recognizes endogenous levels of total PA28α protein. This antibody does not cross-react with either PA28β/PSME2 or PA28γ/PSME3. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3) (1,2). PA28α and PA28β form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28α/β complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2). PA28γ exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28γ complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Western blot analysis of extracts from various cell lines using PA28α (D1C10) Rabbit mAb. Western blot检测多种细胞提取物。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PA28α (hPA28α-Myc/DDK, +), full-length human PA28β (hPA28β-Myc/DDK, +), or full-length human PA28γ (hPA28γ-Myc/DDK, +), using PA28α (D1C10) Rabbit mAb (upper) or DYKDDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (lower). Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged人全长PA28α (hPA28α-Myc/DDK, +)、人全长PA28β (hPA28β-Myc/DDK, +)或人全长PA28γ (hPA28γ-Myc/DDK, +)293T细胞，采用PA28α (D1C10) Rabbit mAb (upper)或DYKDDDDK Tag Antibody（与Sigmas Anti-FLAG® M2 Antibody结合相同的表位）#2368 (lower)。 |
