| 货号 | 8456S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | Pan-Actin (D18C11) Rabbit mAb recognizes endogenous levels of total actin protein (all isoforms). |
| 使用方法 | WB(1:1000) IHC-P (1:200) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from various cell lines using Pan-Actin (D18C11) Rabbit mAb.使用Pan-Actin (D18C11)Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human heart using Pan-Actin (D18C11) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照肽（左）或抗原特异性肽（右）存在的条件下，使用Pan-Actin (D18C11)Rabbit mAb对石蜡包埋的人乳腺癌组织进行免疫荧光分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Pan-Actin (D18C11) Rabbit mAb.使用Pan-Actin (D18C11)Rabbit mAb对石蜡包埋的人结肠癌组织进行免疫组化分析。 | |
Confocal immunofluorescent analysis of HeLa cells using Pan-Actin (D18C11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Pan-Actin (D18C11)Rabbit mAb（绿色）对HeLa细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). |
