| 货号 | 12055S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PANK4 (D8N2C) Rabbit mAb recognizes endogenous levels of total PANK4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Panthothenate kinase (PANK) is an enzyme that is responsible for catalyzing the first step in coenzyme A (CoA) synthesis (1-4). There are four human PANK genes (PANK1-4) (1-4). PANK4 is ubiquitously expressed with higher expression levels seen in muscle (1,2). PANK4 expression is elevated in rat skeletal muscle upon glucose challenge (2). There is evidence that rat PANK4 colocalizes with pyruvate kinase M2 (PKM2) in vitro (2). PANK4 may also play a protective role in beta-cell apoptosis by lowering the levels of pro-caspase-9 (3). Research studies have shown that mutations in the PANK2 gene are associated with Neurodegeneration with Brain Iron Accumulation (NBIA), formerly known as Hallervorden-Spatz syndrome (1,4). Expression of hPANK4 in a Drosophila model of NBIA rescues the phenotype with the exception of infertility (4). |
| 存放说明 | -20C |
| 计算分子量 | 82 |
Western blot analysis of extracts from various cell lines and tissues using PANK4 (D8N2C) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为PANK4 (D8N2C) Rabbit mAb兔单抗。 | |
Immunoprecipitation of PANK4 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PANK4 (D8N2C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PANK4 (D8N2C) Rabbit mAb. 免疫沉淀方法检测HeLa细胞提取物PANK4，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或PANK4 (D8N2C) Rabbit mAb 兔单抗(泳道3)。泳道1为10%对照。使用PANK4 (D8N2C) Rabbit mAb兔单抗进行Western blot分析。 |
