| 货号 | 4545S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-P/IF-IC/F |
| 目标/特异性 | Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. |
| 使用方法 | WB(1:1000) IHC-P (1:500) F (1:50) IF-IC (1:400) IF-P (1:50) |
| 供应商 | CST |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 计算分子量 | 46-58 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.使用Pan-Keratin (C11) Mouse mAb，共聚焦免疫荧光观察Pan-Keratin (C11)蛋白在人源乳腺癌组织石蜡切片中定位。 | |
Flow cytometric analysis of MCF7 cells, using Pan-Keratin (C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).与非特异阴性control antibody (红色)比较，使用Pan-Keratin (C11) Mouse mAb (蓝色)标记，流式细胞仪分析MCF7细胞。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.使用Pan-Keratin (C11) Mouse mAb，共聚焦免疫荧光观察Pan-Keratin (C11)蛋白在人源前列腺癌组织石蜡切片中定位。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.使用Pan-Keratin (C11) Mouse mAb，共聚焦免疫荧光观察Pan-Keratin (C11)蛋白在人源结肠癌组织石蜡切片中定位。 | |
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.使用Pan-Keratin (C11) Mouse mAb，共聚焦免疫荧光观察Pan-Keratin (C11)蛋白在人源转移上皮癌（膀胱）组织石蜡切片中定位。 | |
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.使用Pan-Keratin (C11) Mouse mAb，免疫组化分析H358 xenograft石蜡切片。 | |
Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.使用Pan-Keratin (C11) Mouse mAb，免疫印迹（Western Blot）分析不同细胞中Pan-Keratin (C11)蛋白水平。 | |
Confocal immunofluorescent image of paraffin-embedded human colon carcinoma labeled with Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用Pan-Keratin (C11) Mouse mAb (绿色)标记，共聚焦免疫荧光观察Pan-Keratin (C11)蛋白在人源结肠癌组织石蜡切片中定位。蓝色= DRAQ5™ (DNA荧光染料)。 | |
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). |
