| 货号 | 91137S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The pannexin family (pannexin-1, -2, and -3; PANX1-3) of gap junction proteins has homology to the invertebrate innexins and display distinct expression patterns (1). Pannexin-1 is widely expressed, with highest expression in the heart, brain, skeletal muscle, testis, and ovary (1,2). Pannexin-2 is predominately expressed in the brain (1,2) and pannexin-3 is found within the skin and connective tissues (1,3). Connexin family gap junction proteins form hemichannels that align adjacent cells, creating functional intercellular channels that are permeable to ions and small molecules. In contrast, pannexin proteins may not function as gap junction proteins since pannexins on adjacent cells may not align to form complete channels (3). These pannexin “hemichannels” may play a role in inflammation, apoptosis, and neuronal signaling by allowing permeability of ions, ATP, and potentially other small molecules into the extracellular space (4-6). Pannexin-1 can be activated by effector caspases (caspase-3 and -7), which leads to release of signal molecules that promote phagocytosis of apoptotic cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 45-55, 19 |
Western blot analysis of extracts from various cell lines using Pannexin-1 (D9M1C) Rabbit mAb. | |
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Etoposide #2200 (25 μM, overnight; +), using Pannexin-1 (D9M1C) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human pannexin-1 protein (hPANX1; +), using Pannexin-1 (D9M1C) Rabbit mAb. | |
Immunoprecipitation of pannexin-1 from PANC-1 cell extracts. Lane is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Pannexin-1 (D9M1C) Rabbit mAb. Western blot was performed using Pannexin-1 (D9M1C) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG heavy chain. |
