| 货号 | 6976S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PAR2 (D61D5) Rabbit mAb recognizes endogenous levels of total PAR2 protein. The major band detected runs at 32 kDa, the higher molecular weight smear is due to glycosylation. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | PAR2 (F2RL1) belongs to the PAR (Protease-activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through N-terminal cleavage of the receptor by a serine protease such as thrombin, trypsin, or matrix metalloproteinases (1,2). This cleavage exposes the ‘tethered-ligand’ fragment of the receptor, which binds to a second extracellular loop of the receptor and leads to receptor activation. PAR2 is specifically activated by trypsin or trypsin-like proteases. Activated PAR2 stimulates phosphoinositide hydrolysis and calcium mobilization, interacts with β-arrestin, and eventually leads to ERK activation (3). PAR2 expression and activation are mainly associated with inflammatory diseases (3), but may also play a role in cancer development (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with human PAR2 (+), using PAR2 (D61D5) Rabbit mAb. 使用PAR2 (D61D5) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析COS-7细胞中PAR2蛋白水平，细胞分为未转染(-)或转染人源PAR2(+)。 | |
Western blot analysis of extracts from HCT 116 and Hep G2 cells using PAR2 (D61D5) Rabbit mAb. 使用PAR2 (D61D5) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析HCT 116和HepG2细胞中PAR2蛋白水平。 |
