| 货号 | 13295S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Pan-TEAD (D3F7L) Rabbit mAb recognizes endogenous levels of total TEAD proteins. This antibody has been shown to recognize TEAD 1, 2, 3 and 4 in transfected cell extracts. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned off, YAP is phosphorylated and translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5). |
| 存放说明 | -20C |
| 计算分子量 | 50, 53, 55, 60 |
Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using Pan-TEAD (D3F7L) Rabbit mAb. 对COS－7细胞转染表达Myc/DDK标签的全长人TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK标签的全长人TEAD2 (hTEAD2-Myc/DDK;+)，Myc/DDK标签的全长人 TEAD3 (hTEAD3-Myc/DDK;+)或Myc/DDK标签的全长人TEAD4 (hTEAD4-Myc/DDK;+)后，获取细胞提取物，使用Pan-TEAD (D3F7L) Rabbit mAb兔单抗进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Pan-TEAD (D3F7L) Rabbit mAb. 使用Pan-TEAD (D3F7L) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of TEAD proteins from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Pan-TEAD (D3F7L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Pan-TEAD (D3F7L) Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或Pan-TEAD (D3F7L) Rabbit mAb 兔单抗(lane 3)对COS-7细胞提取物中的TEAD蛋白进行免疫沉淀分析。Lane 1是10%上样量。使用Pan-TEAD (D3F7L) Rabbit mAb兔单抗进行western blot分析。 | |
