| 货号 | 3086S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PASK (C70B2) Rabbit mAb detects endogenous levels of total PASK protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | PASK is a serine/threonine kinase that contains two PAS (Per-Arnt-Sim) domains (1). Its kinase activity is up-regulated by autophosphorylation of the activation loop within its catalytic domain and is inhibited in cis by one of the PAS domains (1). Studies found that the yeast homolog of PASK phosphorylates and inhibits two enzymes required for glycogen biosynthesis: UDP-glucose pyrophosphorylase and glycogen synthase, resulting in the decrease of carbohydrate storage (2). Further studies showed that increased glucose levels activate PASK activity and enhance its expression in pancreatic β-cells (3). PASK is essential for the glucose-stimulated expression of preproinsulin and Pdx1, suggesting its role in the regulation of genes involved in pancreatic β-cell functions (3). PASK was shown to be critical for glucose-stimulated insulin secretion in pancreatic β-cells (4). The absence of PASK also protects animals from obesity and insulin resistance when they are fed a high-fat diet (4). These findings suggest that PASK functions as an important metabolic sensor in various cells (4). |
| 存放说明 | -20C |
| 计算分子量 | 170 |
Western blot analysis of extracts from NIH/3T3, L6 and HepG2 cells using PASK (C70B2) Rabbit mAb. 对NIH/3T3, L6和HepG2细胞裂解液使用PASK (C70B2) Rabbit mAb兔单抗进行Western blot分析。 |
