| 货号 | 12940S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PCK1 (D12F5) Rabbit mAb recognizes endogenous levels of total PCK1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Phosphoenolpyruvate carboxykinase 1 (PCK1, PEPCK1 or PEPCK-C) is a cytosolic enzyme responsible for the conversion of oxaloacetate to phosphoenolpyruvate (1). PCK1 is involved in controlling the rate-limiting step of gluconeogenesis in the liver, which generates glucose from non-carbohydrate substrates such as lactate and glycerol (2). The deacetylase SirT1 stimulates transcription of PCK1 and glucose-6-phosphatase to activate gluconeogenesis (3). Depending on nutritional state, Stat3 can inhibit PCK1 and glucose-6-phosphatase expression and suppress gluconeogenesis (4). Relatively high glucose concentration can result in acetylation of PCK1 by P300 acetyltransferase, promoting an interaction between PCK1 and the E3 ligase UBR5 that leads to the PCK1 destabilization (5). |
| 存放说明 | -20C |
| 计算分子量 | 63 |
Western blot analysis of extracts from various cell lines using PCK1 (D12F5) Rabbit mAb. 使用PCK1 (D12F5) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PCK1 (hPCK1-Myc/DDK; +), using PCK1 (D12F5) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). 模拟转染(-)或转染了表达Myc/DDK标记full-length human PCK1 (hPCK1-Myc/DDK; +)质粒的293细胞，使用PCK1 (D12F5) Rabbit mAb (上), DYKDDDDK Tag (9A3) Mouse mAb #8146 (中), 或β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下)对293细胞提取物进行western blot分析。 |
